| Registro completo |
| Provedor de dados: |
Rev. Bras. Frutic.
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| País: |
Brazil
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| Título: |
In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)
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| Autores: |
Soares,Taliane Leila
Jesus,Onildo Nunes de
Santos-Serejo,Janay Almeida dos
Oliveira,Eder Jorge de
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| Data: |
2013-12-01
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| Ano: |
2013
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| Palavras-chave: |
Passion fruit
Pollen germination
Histochemical analysis
Wild passion fruit
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| Resumo: |
The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO3) 4H2O, 0.02% of Mg(SO4 ).7H2O, 0.01% of KNO3, 0,01% of H3BO3, 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452013000400023
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| Editor: |
Sociedade Brasileira de Fruticultura
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| Relação: |
10.1590/S0100-29452013000400023
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| Formato: |
text/html
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| Fonte: |
Revista Brasileira de Fruticultura v.35 n.4 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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